Splicing Factor 1 anticorps
Aperçu rapide pour Splicing Factor 1 anticorps (ABIN2462368)
Antigène
Voir toutes Splicing Factor 1 (SF1) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Antibody is purified by peptide affinity chromatography method.
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Immunogène
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SF1.
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Indications d'application
- SF1 antibody can be used for detection of SF1 by ELISA at 1:62500. SF1 antibody can be used for detection of SF1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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Concentration
- 1 mg/mL
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Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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Conseil sur la manipulation
- As with any antibody avoid repeat freeze-thaw cycles.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store SF1 antibody at -20 °C.
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- Splicing Factor 1 (SF1)
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Autre désignation
- SF1
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Sujet
- SF1 contains 1 KH domain and 1 CCHC-type zinc finger. It is necessary for the ATP-dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. It may act as transcription repressor.
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Poids moléculaire
- 68 kDa, 69 kDa
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ID gène
- 7536
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NCBI Accession
- NP_004621
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UniProt
- Q15637
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Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location
Antigène
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